Composite

Part:BBa_K5246043:Design

Designed by: Edgaras Zaboras   Group: iGEM24_Vilnius-Lithuania   (2024-09-25)


Caulobacter crescentus CB2/CB2A HfsE-HfsJ-HfsG-HfsH-HfsK-HfsL Polysaccharide tetrad assembly


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3690
    Illegal XbaI site found at 4450
    Illegal PstI site found at 4539
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3690
    Illegal PstI site found at 4539
    Illegal NotI site found at 619
    Illegal NotI site found at 3444
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3690
    Illegal BglII site found at 2280
    Illegal BglII site found at 4453
    Illegal BamHI site found at 2428
    Illegal XhoI site found at 2647
    Illegal XhoI site found at 5268
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3690
    Illegal XbaI site found at 4450
    Illegal PstI site found at 4539
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3690
    Illegal XbaI site found at 4450
    Illegal PstI site found at 4539
    Illegal NgoMIV site found at 758
    Illegal NgoMIV site found at 1389
    Illegal NgoMIV site found at 2001
    Illegal NgoMIV site found at 3099
    Illegal NgoMIV site found at 3748
    Illegal NgoMIV site found at 3772
    Illegal NgoMIV site found at 3776
    Illegal AgeI site found at 1088
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NA


Source

BBa_J64997, BBa_K4165062, BBa_K2621038 and BBa_K731721 are parts of pRSF-Duet-1 backbone. All the other parts are from C. crescentus CB2(DSM 4727)/CB2A(DSM 25117) strains.

References